General Western Blot Reagents
Abcam Plasminogen activator Inhibitor Type 1 Human Chromogenic Activity Assay Kit
Plasminogen activator Inhibitor Type 1 Human Chromogenic Activity Assay Kit
The product is subject to the following: Abcam Restricted Use Statement
ABclonal Technology CYP1A2 Rabbit pAb
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The protein encoded by this gene localizes to the endoplasmic reticulum and its expression is induced by some polycyclic aromatic hydrocarbons (PAHs), some of which are found in cigarette smoke. The enzymes endogenous substrate is unknown, however, it is able to metabolize some PAHs to carcinogenic intermediates. Other xenobiotic substrates for this enzyme include caffeine, aflatoxin B1, and acetaminophen. The transcript from this gene contains four Alu sequences flanked by direct repeats in the 3 untranslated region.
Alkali Scientific PVDF Membrane Stripping Buffer 4X Solution, 1 Liter
PVDF Membrane Stripping Buffer 4X Solution is designed to effectively remove bound antibodies from PVDF membranes after Western blotting, allowing for the reuse of membranes in multiple probing experiments. The 4X concentrated solution is ideal for stripping and reprobing membranes with different antibodies, providing a cost-effective way to maximize the utility of membrane samples. This buffer maintains membrane integrity during the stripping process, ensuring high-quality results for subsequent analyses. The 1-liter solution is perfect for use in laboratories where multiple antibody strips and reprobes are routinely performed, saving both time and materials in experimental setups.
Bioworld Blot Stripping Buffer II (4X), 500 mL
Product DescriptionBlot stripping buffer II (4x) contains enough liquid concentrate to make 4L of 1X solution. Product is 0.2M glycine and has a pH value of 2.5.To use this product, place the membrane into stripping buffer for 30-45 minutes with gentle agitation. Buffer should be heated to approximately 50°C for incubation. Wash membrane 3 times with TBST or PBST before reprobing.Product is ultra pure grade.
Bioworld Nitrocellulose Stripping Buffer (10X), 500 mL
Product DescriptionNitrocellulose stripping buffer (10X) is used for hybridization of probes to nucleic acids. It is ideal for removal of secondary antibody after detection of highly abundant targets. It is also used for nucleic acid and protein related blotting.This buffer contains 0.5X SSC and 100 mM EDTA at pH 8.0. To strip the membrane, dilute buffer to 1X in water. Warm solution to 50-70°C and perform 2 to 3 changes (15 minutes each) of boiling stripping buffer.
Alkali Scientific Blot Stripping Buffer 4X Solution, 500 Milliliters
Blot Stripping Buffer 4X Solution is a powerful reagent for the efficient removal of antibodies or proteins from membranes, making them suitable for reuse in subsequent Western blot experiments. This 500mL bottle provides a large quantity of buffer ideal for labs conducting high-throughput analysis or multiple rounds of probing. The buffer’s 4X concentration ensures that it effectively strips the membrane while maintaining its integrity for future use. It is commonly employed in protein analysis experiments, where stripping and re-probing the same membrane for different target proteins are necessary, offering a cost-effective solution for repeated analysis.
Alkali Scientific PVDF Membrane Stripping Buffer 4X Solution, 500 Milliliters
PVDF Membrane Stripping Buffer 4X Solution is designed for Western blotting applications where stripping and re-probing of PVDF membranes are required. This 4X concentrated solution effectively removes bound antibodies from the membrane without damaging the protein or membrane integrity. It is ideal for stripping multiple antibodies from the same blot for re-probing with different antibodies. The concentrated formulation allows for the use of smaller volumes while maintaining effective stripping. This buffer is essential for researchers performing multiple antibody assays on the same PVDF membrane, saving time and resources in protein analysis.
Bioworld Blot Stripping Buffer (4X), 500 mL
Product DescriptionBlot stripping buffer (4x) contains enough liquid concentrate to make 1L of 1X solution. It contains 250 mM Tris and 8% SDS. At 25°C, it has a pH value of 6.8.To use this product, dilute it 4 fold with deionized (DI) water and add 0.6% of β-Mercaptoethanol (beta ME). Wash the membrane in this buffer and incubate at 65°C for 30 minutes under constant agitation. Wash the membrane 5X for 5 minutes in PBS-tween20 or TBS-tween20.Product is an irritant hazard.
Bioworld Nylon Membrane Stripping Buffer (10X), 250 mL
Product DescriptionNylon Membrane Stripping Buffer (10X) is a liquid used for the hybridization of probes to nucleic acids. Buffer contains Tris HCL at pH 8.0 (1mM), EDTA (100mM), and Denhardt’s solution (1X). To use this product, dilute at 10-fold with sterile deionized water and strip the membrane by 2 hours of incubation at 75°C.
Biotang Inc Striping Buffer3 (4X) Glycine 0.4M SDS 0.2% Tween-20 2% pH 2.5, 250 ml
Striping Buffer3 (4X) Glycine 0.4M SDS 0.2% Tween-20 2% pH 2.5, 250 ml.